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High Sensitivity DNA Rapid Isothermal Amplification Kit Basic

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Amp-Future (Changzhou) Biotech Co., Ltd.

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High Sensitivity DNA Rapid Isothermal Amplification Kit Basic

Name: High Sensitivity DNA Rapid Isothermal Amplification Kit Basic
Brand: Amp-future bio
Price: 3.5 USD

Brand Name : Amp-future bio

Model Number : WLB8201KIT

Certification : ISO9001

Place of Origin : China

MOQ : 16T

Price : USD$3.5/T

Payment Terms : T/T, MoneyGram

Supply Ability : 100000T/month

Delivery Time : 7days

Packaging Details : 48T/box

Application : Detection and Quantification of Nucleic Acids

Certifications : ISO

Detection Method : AGE

Number of Reactions : 48T

Product Name : DNA Isothermal Amplification Kit Basic

Sample Type : Blood,Saliva

Sensitivity : High

Storage Temperature : -20°C

Type : Amplification Kit

Detection Range : DNA

Kit Components : Buffers,enzymes,dNTPS

Reaction Volume : 50μL

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`Product Detail`

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3' end of the primer to start the chain extension.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit Basic
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(Basic type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

Colloidal gold probe:Require a sequence of 46-52nt in length

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

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